| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Khoury, Rita M (14)
|
AMHD |
2:24.21 |
2:30.26 |
6.05 |
| 2 |
Anderson, Ariana A (13)
|
AMPRA |
2:29.38 |
2:31.92 |
2.54 |
| 3 |
LaRoche, Francesca L (14)
|
AMWHAT |
2:32.91 |
2:35.69 |
2.78 |
| 4 |
Massella, Maggie E (14)
|
AMSRST |
2:38.46 |
2:37.19 |
-1.27 |
| 5 |
Van Deusen, Gretchen P (13)
|
AMGPAC |
2:36.52 |
2:44.46 |
7.94 |
| 6 |
Wienand, Paige L (13)
|
AMMMSA |
2:51.25 |
2:47.25 |
-4.00 |
| 7 |
Harris, Emma R (13)
|
AMPTAC |
2:45.44 |
2:49.51 |
4.07 |
| 8 |
DeVito, Mia (13)
|
AMCSC |
2:51.84 |
2:50.01 |
-1.83 |
| 9 |
Johnson, Olivia J (14)
|
AMPTAC |
2:46.79 |
2:50.74 |
3.95 |
| 10 |
Tucker, Angela M (14)
|
AMCSC |
2:56.90 |
2:54.38 |
-2.52 |
| 11 |
Mushinsky, Ava E (14)
|
AMHD |
3:26.98 |
2:57.23 |
-29.75 |
| 12 |
Strasser, Scarlet Sophia F (13)
|
AMMMSA |
2:55.99 |
2:57.61 |
1.62 |
| 13 |
Ferderbar, Camryn R (14)
|
AMHD |
3:38.98 |
2:58.07 |
-40.91 |
| 14 |
Smith, Kaileigh M (13)
|
AMGPAC |
2:47.11 |
2:58.63 |
11.52 |
| 15 |
Michael, Lauren C (14)
|
AMHD |
3:23.23 |
3:02.82 |
-20.41 |
| 16 |
Poniatowski, Shelby A (13)
|
AMNHA |
3:01.66 |
3:08.89 |
7.23 |
| 17 |
Polliard, Abbi A (13)
|
AMCSC |
2:56.78 |
3:10.47 |
13.69 |
| 18 |
Hutchison, Isabella N (13)
|
AMCSC |
3:37.89 |
3:11.90 |
-25.99 |
| 19 |
Chelli, Kaysia M (13)
|
AMSASC |
3:06.72 |
3:13.88 |
7.16 |
| 20 |
Burton, Elizamay J (13)
|
AMHD |
3:55.98 |
3:31.79 |
-24.19 |
|
Poliziani, Alyssandra M (14)
|
AMSASC |
2:38.87 |
DQ |
- |
|
Reed, Ella A (14)
|
AMJCCS |
2:59.73 |
NS |
- |
|
Spiess, Emma E (13)
|
AMHD |
2:43.19 |
NS |
- |
|
Albertson, Jessica A (13)
|
AMPRA |
3:02.68 |
NS |
- |
|
Kandor, Sydney L (13)
|
AMPRA |
3:01.26 |
NS |
- |
|
Ramanathan, Mira S (13)
|
AMPRA |
3:29.11 |
NS |
- |
|
Croushore, Gracie P (14)
|
AMUNAT |
2:57.82 |
NS |
- |